Beatriz Fucuda Nascimento
Universidade de São Paulo, Brazil
Title: Phenolic compounds present on the leaves of Eugenia uniflora identify by HPLC - IR
Biography
Biography: Beatriz Fucuda Nascimento
Abstract
Eugenia uniflora L. are popularly known as “brazilian cherry” and it is consumed by brazilian people, known as antihypertensive, antirheumatic, diuretic, antipyretic astringent and for the treatment of digestive disorders. Studies report an anti-inflammatory effect throught the infusion of dry leaves. E. uniflora belongs to Myrtaceae family. This family presents 132 genus and 5.671 species, where these species are recognized by the presence of essencial oils, flavonoids, phenolic compounds, alkaloids and saponins.The plant material of E. uniflora was collected on Tropical Municipal Park Francisco Affonso de Mello, located in Mogi das Cruzes, São Paulo – Brazil. After drying and milling, the leaves of E. uniflora was extracted by percolation in 80% ethanol solution. A portion of 20 g of the dryed extract was resuspended in ethyl acetate and water 1:1 (v/v). The fraction ethyl acetate was evaporated, obtaining 3,3 g. This fraction was dissolved in 12 mL of methanol/water 80:20 (v/v) and after centrifugation, the superior part was fractionated in column LH-20 (methanol as mobile phase), getting 108 fraction of 5 mL each. Analyses made with TLC using as mobile phase chloroform/methanol/water 80:18:2 (v/v), allowed grouping the fractions of same chromatographic profile. The study of the fractions 16-28, 29-32 and 33-39 and analyses in scale semipreparative resulted in the isolation of 7 compouds. The analyses of the spectroscopy datas of RMN 1H, 13C, bidimentional experients of HSQC, HMBC e COSY, allowed to identify the compounds as being gallic acid, ethyl gallate, 3-galloyl quinic acid, 5-galloyl quinic acid, quercetin-3-O-rhamnopyranoside and miricetin-3-O-rhamnopyranoside. The ethyl gallate was described by the first time in E. uniflora.