Maryam Salimi
Tehran Province Water and Waste water Company, Iran
Title: :Optimization of dispersive liquid-liquid microextraction (DLLME) method for determination of 2, 4-D and MCPA in water by HPLC with using Box-Behnken design
Biography
Biography: Maryam Salimi
Abstract
This paper describes the optimization of DLLME method for determination of Chlorophenoxy herbicides including 2, 4 D and MCPA by high-performance liquid chromatography (HPLC). For DLLME, a mixture of extraction solvent (Ethyl acetate, 0.35 ml for 2,4 D and 0.6 ml for MCPA) and dispersive solvent (acetonitrile, 0.65 ml for 2,4 D and 0.4 ml for MCPA), were rapidly injected into water sample for the formation of cloudy solution. The analysis was performed on a high-performance liquid chromatography (HPLC) system with a C18 column (250×4.6 mm, i.e.), and mobile phase contain of 0.05 M H3 PO4/acetonitrile (90:10) to 100 acetonitrile in 30 minutes. The flow rate was 1 mL min−1, and the chromatogram was monitored at a wavelength of 280 nm. In this study, the effects of concentration of sample, pH, volume of solvent extraction, extraction time in the distributed liquid-liquid extraction have been examined and optimal conditions were obtained using the Box-Behnken